Effect of uvrY on AI-2 accumulation and expression of luxS::lacZ fusion.
(A) AI-2 accumulation in the supernatant was assayed by Vibrio harveyi reporter assay in SM1005 (luxS::lacZ), SM1007 (ΔuvrY luxS::lacZ) and SM1010 (ΔuvrY/puvrY luxS::lacZ) for a period of 24 hours. (B) Expression of the fusion in the strains was monitored by β-galactosidase assay for 24 hours. Dotted lines indicate growth of corresponding bacterial strains. The graphs represent mean of three experiments. Asterisk marks are provided for a representative data point used for calculation of significance.