Effect of chemoresistance on in vivo MIF-CXCR4 axis and Bcl-2 expression.

(A) Immunohistochemical staining for indicated proteins expression in vivo tumor aggressive phenotype. H&E staining revealed tumor growth among all groups of vessel numbers. (B) The positive stained area was evaluated from three randomly selected observation fields of each sections. Quantitative of immunohistochemical proteins C-X-C chemokine receptor type 4 (CXCR-4), MIF and Bcl-2 by average integrated optical density (AIOD). Positive stained area was evaluated from three randomly selected observation fields of each section. Data were expressed as means ± SEM (n = 8/group). * p < 0.05, compared with control group, parent group. Magnification ×400.