Effect of borrelidin on the growth of WT, ThrRS/NEO and ThrRS/NEO[pThrRS⁺] parasites.

(A) and (B) Inhibition profile of borrelidin for the promastigote (A) and intracellular amastigote (B) growth of WT parasites. Percentage parasite survival was plotted against different concentrations of borrelidin. (C) and (D) The leishmanicidal effect of borrelidin was checked on promastigotes (C) and amastigotes (D) of WT, ThrRS/NEO and ThrRS/NEO[pThrRS⁺] parasites. The mean IC₅₀ values were calculated for borrelidin and plotted as bar graphs. (E) The effect of miltefosine on WT, ThrRS/NEO and ThrRS/NEO[pThrRS⁺] promastigotes. The bar graphs represent mean ± SD with n = 3. * p < 0.05.