Effect of apical plasma membrane LLC-PK1 exosomes with GAPDH depleted activity on ENaC activity in Xenopus 2F3 cells.
(A) GAPDH activity was measured after lysing apical plasma membrane LLC-PK1 exosomes treated with the potent and selective GAPDH inhibitor Heptelidic acid (Koningic acid). (B) Representative single-channel patch clamp recording from the control and treated group. (C-E) Summary of all single channel data. The summary bar graph in (C) shows ENaC activity, NPo. The summary bar graph in (D) shows the number of channels, N. The summary bar graph in (E) shows the open probability, Po for the control and treated groups. Data is presented as the mean ± s.e. and the number of patches is indicated above each group.