ELSD chromatograms for the methanolic extract before and after enrichment, and its fractionation by preparative chromatography.

(A) UHPLC chromatogram of the crude methanolic extract exhibiting mainly unretained polar metabolites. (B) UHPLC metabolite profiling after enrichment by VLC to remove very polar residues. (C) Preparative chromatography for the fractionation of the enriched extract highlighting the isolation of constituent 1.