Drug response to anticancer agents in MCTs with MRC-5.

(a) Staining assay of live and dead cells with use of calcein AM (live cells) and ethidium bromide (dead cells) (b) Percentages of dead cells based on the measurements of the fluorescence intensities of live/dead cells using ImageJ on day 2 and 4 after the initiation of drug administration. (c) Cell viability was performed using the CCK–8 assay kit and was measured at 450 nm using a microplate reader on day 2 and 4 after the initiation of drug administration. (d) Annexin V and PI staining were used to identify the apoptotic cell death after drug administration. p < 0.05(*) and p < 0.005(**).