ppat.1008074.g004.tif (315.92 kB)
Differentiation drives reactivation of clonally-expanded, replication-competent HIV-1.
figure
posted on 2019-10-14, 17:27 authored by Elizabeth R. Wonderlich, Krupa Subramanian, Bryan Cox, Ann Wiegand, Carol Lackman-Smith, Michael J. Bale, Mars Stone, Rebecca Hoh, Mary F. Kearney, Frank Maldarelli, Steven G. Deeks, Michael P. Busch, Roger G. Ptak, Deanna A. KulpaP6-PR-RT single-genome sequencing[78] was performed on HIV-1-Gag+ culture supernatants from dQVOA on NIH participant 2–20 (n = 2), NIH participant 2–37 (n = 1), and RAVEN participant 2147-R (n = 1). Circles represent single RNA genomes in the supernatants. Sequences obtained from different wells are shown in different colors. Identical sequences across different wells may result from latency reversal of infected cell clones. Sequences within wells that are different by a single nucleotide or two from a large rake of identical sequences are likely the result of RT error upon viral replication in the QVOA wells rather than from induction of different proviral variants.
