Fig 1.tif (783.2 kB)
Diagram of sequenced adaptor-antibody-adaptor constructs.
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posted on 2017-05-15, 17:50 authored by Marco Fantini, Luca Pandolfini, Simonetta Lisi, Michele Chirichella, Ivan Arisi, Marco Terrigno, Martina Goracci, Federico Cremisi, Antonino CattaneoA) scFv library (gray), comprising heavy (VH) and light chain (VL) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). B) VH nanobody library (gray), comprising heavy chain (VH) Complementary Determining Regions (CDR), was ligated to adapters (light green and pink) harbouring Illumina P5 and P7 flowcell hybridization sequences (green and red). The forward read (R1) uses SBS3 sequencing primer (Illumina), while the reverse read (R2) uses SBS12 primer (Illumina). iS1 and iS2 = index/shifter sequences.
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sample sizeAntibody Librarysequencing errorlibrary elementsintracellular protein interference studiesIllumina platformantibody library complexitybioinformatic analysisantibody libraryantibody library complexity quality assessmenterror compensation Antibody librariesantibody library diversityantibody repertoiresInferring complexityQuantitative evaluationsequence antibody librariesNGS approachantibody library complexity estimateDiversity Estimatortransformation efficiencygeneration sequencingNext-generation sequencing
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