Determination of limit of detection of in vitro assay.

Note 1: Percentage of positive wells from DFA assay (gray bars) and quantification rates (log10) of RABV at 72 h of incubation with duplex RT-qPCR (black line) calculated as the mean from 3 independent experiments. Note 2. For the sample to be considered positive in the DFA test, it must have at least one positive well, while for RT-qPCR positivity, the quantification obtained in RT-qPCR at 72 h p.i. must be above the quantitation limit and higher than that observed at 0 h”.