Deficiency of adipose LPP3 does not impact body size or adipose tissue development in mice on a standard diet.

Body weight of male (A) and female (B) Plpp3fl/fl (fl/fl; open symbols) and AP2-Cre/Plpp3Δ (Δ; closed symbols) mice. Results are graphed as mean ± SD from 4–5 animals/group. Weight in grams of interscapular brown adipose tissue (IBAT), subcutaneous (SUB) white adipose tissue, and liver, and femur length (in mm) in 9-week old male (C) and female (D) Plpp3fl/fl (fl/fl; open bars) and AP2-Cre/Plpp3Δ (Δ; closed bars) mice. Comparisons between genotypes were made by Students t-test. E. Representative histology of Plpp3fl/fl (fl/fl) and AP2-Cre/Plpp3Δ (Δ) subcutaneous white adipose tissue stained with Periodic acid–Schiff (PAS) at 10x (left) and 20x (right) magnification. F. Expression analysis of inflammatory gene expression in visceral fat from Plpp3fl/fl (fl/fl; open bars; n = 5) and AP2-Cre/Plpp3Δ (Δ; closed bars; n = 4) mice on high fat diet. Results are graphed as mean ± SD and data analyzed by two-tailed t-test.