Correlation of RNA-seq and qRT-PCR.

2017-01-12T17:38:11Z (GMT) by Jason E. Cummings Richard A. Slayden

Fragments per kilobase of transcript per million mapped reads (FPKM) for differentially expressed genes and 16s reference gene were converted to Log2 values and plotted against respective qRT-PCR crossing point (Cp) values. Linear regression analysis (dotted line) was run to determine if the correlation coefficient was >0.7. An inverse relationship between Cp and FPKM is an indication of correlation and validation of the RNA-seq data set.