Correlation between PTTG1 and miRNA-186-5p in invasion ability of HTR-8/SVneo cells.

(A) Expression of miRNA-186-5p in HTR-8/SVneo cells after 10 nM siPTTG1 treatment, analyzed by qRT-PCR. (B) Expression of miRNA-186-5p after miR-186-5p mimic or inhibitor treatment analyzed by qRT-PCR. (C) mRNA expression of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (D) Expression and density of PTTG1 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p. (E) Images and numbers of invaded HTR-8/SVneo cells exposed to 30 nM miR-186-5p (original magnification, ×100; scale bar = 80 μm). (F) Expression of MMP-9 and MMP-2 in HTR-8/SVneo cells after treatment with 30 nM miR-186-5p, analyzed by zymography. Values are means ± standard errors. *p < 0.05 and **p < 0.001. U6, β-actin, or GAPDH was used as a loading control.