Copper sensitivity does not correlate with sensitivity to oxidative stress.

Dilutions of the indicated cells were spotted onto (A) YPD containing H₂O₂ or (B) synthetic medium with CuSO₄. A cat1Δ catalase deletion served as a control as it is highly sensitive to oxidative stress, but grew to wild type levels on CuSO₄ media. Conversely, the known copper-sensitive mutant cup1Δ crp1Δ grew well on H₂O₂, but failed to grow on either CuSO₄ concentration. Hog1 pathway strains pbs2Δ, sskΔ 2, and hog1Δ all displayed sensitivity to increasing concentrations of both H₂O₂ and CuSO₄, as did pil1Δ lsp1Δ. sur7Δ was inhibited by copper but grew similar to wild type on H₂O₂ media. Images are representative of two independent experiments performed on different days. Strains used were wild type, DIC185, sur7Δ (YJA11), pil1Δ lsp1Δ (YHXW21-1), ssk2Δ (YLD185-7), pbs2Δ (YLD197-1), hog1Δ (YLD184-3), cat1Δ (MT505-A), and cup1Δ crp1Δ (KC25).