Copper sensitivity does not correlate with sensitivity to oxidative stress.

2019-01-11T18:38:04Z (GMT) by Lois M. Douglas James B. Konopka
<p>Dilutions of the indicated cells were spotted onto (A) YPD containing H<sub>2</sub>O<sub>2</sub> or (B) synthetic medium with CuSO<sub>4</sub>. A <i>cat1Δ</i> catalase deletion served as a control as it is highly sensitive to oxidative stress, but grew to wild type levels on CuSO<sub>4</sub> media. Conversely, the known copper-sensitive mutant <i>cup1Δ crp1Δ</i> grew well on H<sub>2</sub>O<sub>2</sub>, but failed to grow on either CuSO<sub>4</sub> concentration. Hog1 pathway strains <i>pbs2Δ</i>, <i>sskΔ 2</i>, and <i>hog1Δ</i> all displayed sensitivity to increasing concentrations of both H<sub>2</sub>O<sub>2</sub> and CuSO<sub>4</sub>, as did <i>pil1Δ lsp1Δ</i>. <i>sur7Δ</i> was inhibited by copper but grew similar to wild type on H<sub>2</sub>O<sub>2</sub> media. Images are representative of two independent experiments performed on different days. Strains used were wild type, DIC185, <i>sur7Δ</i> (YJA11), <i>pil1Δ lsp1Δ</i> (YHXW21-1), <i>ssk2Δ</i> (YLD185-7), <i>pbs2Δ</i> (YLD197-1), <i>hog1Δ</i> (YLD184-3), <i>cat1Δ</i> (MT505-A), and <i>cup1Δ crp1Δ</i> (KC25).</p>