Confirmation of infectivity in vivo.

Note: (A) mice injected intra-cerebrally with IVS–, 21 days after inoculation showing no clinical signs of rabies; (B) mice injected intra-cerebrally with IVS+, 5 days after inoculation showing specific rabies symptoms, such as ruffled fur, hunched back, slow movements and paralysis; (C) brain DFA of mouse injected intra-cerebrally with IVS–(40x); (D) brain DFA of mouse injected intra-cerebrally with IVS+ (40x); (E) brain TEM of mouse inoculated with IVS–(1 μm); (F) brain TEM of mouse inoculated with IVS+, arrow indicates rabies virus particles (200 nm); (G) brain H&E stain of mouse inoculated with IVS–, basal ganglia with normal cellularity (50x); (H) brain H&E stain of mouse inoculated with IVS+, Purkinje cell with Negri body–arrow (400x).