Confirmation of Ph. sergenti salivary proteins expression in supernatant of COS-7 cell culture using western blot analysis by anti-His antibody.

COS-7 cell lines were transiently transfected with recombinant plasmids encoding for fourteen Ph. sergenti salivary proteins. Forty-eight h after transfection, the supernatants were subjected to western blot analysis using anti-His antibody. Line 1. VR1020-PsSP7 (MW, 30.4 KDa), line 2. VR1020-PsSP9 (17.8 KDa), line 3. VR1020-PsSP20 (46.1 KDa), line 4. VR1020-PsSP42 (39.6 KDa), line 5. VR1020-PsSP52 (32.5 KDa), line 6. VR1020-PsSP54 (18.4 KDa), line 7. VR1020-PsSP40 (39.3 KDa), line 8. VR1020-PsSP41 (37.4 KDa), line 9. VR1020-PsSP14 (20.8 KDa), line 10. VR1020-PsSP15 (18.4 KDa), line 11. VR1020-PsSP73 (16 KDa), line 12. VR1020-PsSP98 (18 KDa), line 13. VR1020-PsSP26 (46.2 KDa), line 14. VR1020-PsSP44 (24.8 KDa), line 15. VR1020.