pntd.0007067.g001.tif (1.71 MB)

Confirmation of Ph. sergenti salivary proteins expression in supernatant of COS-7 cell culture using western blot analysis by anti-His antibody.

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posted on 2019-01-11, 18:37 authored by Elham Gholami, Fabiano Oliveira, Tahereh Taheri, Negar Seyed, Safoora Gharibzadeh, Nasim Gholami, Amir Mizbani, Fatemeh Zali, Sima Habibzadeh, Daniel Omid Bakhadj, Claudio Meneses, Kambiz Kamyab-Hesari, Alireza Sadeghipour, Yasaman Taslimi, Fatemeh khadir, Shaden Kamhawi, Mohammad Ali Mazlomi, Jesus G. Valenzuela, Sima Rafati

COS-7 cell lines were transiently transfected with recombinant plasmids encoding for fourteen Ph. sergenti salivary proteins. Forty-eight h after transfection, the supernatants were subjected to western blot analysis using anti-His antibody. Line 1. VR1020-PsSP7 (MW, 30.4 KDa), line 2. VR1020-PsSP9 (17.8 KDa), line 3. VR1020-PsSP20 (46.1 KDa), line 4. VR1020-PsSP42 (39.6 KDa), line 5. VR1020-PsSP52 (32.5 KDa), line 6. VR1020-PsSP54 (18.4 KDa), line 7. VR1020-PsSP40 (39.3 KDa), line 8. VR1020-PsSP41 (37.4 KDa), line 9. VR1020-PsSP14 (20.8 KDa), line 10. VR1020-PsSP15 (18.4 KDa), line 11. VR1020-PsSP73 (16 KDa), line 12. VR1020-PsSP98 (18 KDa), line 13. VR1020-PsSP26 (46.2 KDa), line 14. VR1020-PsSP44 (24.8 KDa), line 15. VR1020.

Confirmation of Ph. sergenti salivary proteins expression in supernatant of COS-7 cell culture using western blot analysis by anti-His antibody.

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