Comparison of various Hydra anesthetics.

A. Comparisons of the same animal after 15 min and 60 min of anesthetic exposure. i. 1 mM linalool, ii. 0.04% heptanol, iii. 2% urethane, iv. 0.1% chloretone. Scale bars: 1 mm. B. Maximum intensity projections of GCaMP6s animals at 60x magnification in each anesthetic. Scale bars: 10 μm. C. Maximum intensity projections of two-channel images of watermelon animals stained with Hoechst nuclear dye at 60x magnification. GFP channel, DAPI channel, and merge (overlay) shown for each anesthetic. Scale bars: 10 μm.