Comparison of the <i>S</i>. <i>bovis</i> proteome with other schistosome species.

<p>A) <i>S</i>. <i>bovis</i> centred Venn diagram. The outer ring represents the 11,631 proteins predicted from the <i>S</i>. <i>bovis</i> genome, while the inner 3-way Venn diagram shows the subset of predicted <i>S</i>. <i>bovis</i> proteins that were present in the genomes of <i>S</i>. <i>haematobium</i> (Egypt isolate), <i>S</i>. <i>mansoni and S</i>. <i>japonicum</i>, respectively, based on protein sequence homology. The colours of the inner 3-way Venn diagram correspond to the number of proteins in each intersection from low (light grey) to high (dark blue). A <i>Schistosoma</i> core-proteome of 8,105 proteins was identified as those proteins that had sequence homology matches across all four species. 95 predicted proteins were unique for <i>S</i>. <i>bovis</i>. B) Sequence identity of <i>S</i>. <i>bovis</i> proteins and orthologous proteins from other schistosome species. Proteins of <i>S</i>. <i>bovis</i> and <i>S</i>. <i>haematobium</i> were significantly more conserved (p<10<sup>−15</sup>, two-tailed t-test) than the proteomes of <i>S</i>. <i>bovis-S</i>.<i>-mansoni</i> and <i>S</i>. <i>bovis-S</i>. <i>japonicum</i>. C) Sequence conservation of <i>S</i>. <i>bovis</i> tegument proteins and secreted peptides. <i>S</i>. <i>bovis</i> proteins were mapped to the <i>S</i>. <i>haematobium</i> genome sequence using Exonerate. Tegument proteins and secreted peptides showed an average sequence identity of 98.9% and 97.8%, respectively. All tegument proteins and secreted peptides included in this analysis were shared by both species. D) Analysis of the <i>Schistosoma</i> pan-genome. Proteomes of four <i>Schistosoma</i> species were concatenated, clustered into orthologous groups and mapped to each <i>Schistosoma</i> genome using Exonerate. Colours depict the number of proteins in each intersection from low (light orange) to high (dark orange). A pan-genome of 13,297 orthologous groups and a <i>Schistosoma</i> core-genome of 9,851 proteins were identified.</p>