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Common posterior Distal Tip Cell (DTC) trajectories in ngat-1(ev821) mutant animals.

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posted on 2017-08-17, 17:57 authored by Joseph Veyhl, Robert J. Dunn, Wendy L. Johnston, Alexa Bennett, Lijia W. Zhang, James W. Dennis, Harry Schachter, Joseph G. Culotti

Lateral view of the posterior gonad arm in L4 hermaphrodite animals by D.I.C. microscopy. In all panels, anterior is left, dorsal is up. (A) The 2 DTCs are born in ventral mid-body (in position of future vulva marked by triangle) and normally migrate post-embryonically in 3 phases [(1) thru (3)] to shape the developing U-shaped gonad arms. (B) Some ngat-1(ev821) animals grown at 25°C have a normal posterior DTC migration as in panel A, while many have an unc-6 mutant-like phase 2 failure with phases 2 and 3 executed on the ventral side (panel B), while others (panel C) show a partial return to mid-body before migrating dorsally. To the right of panels B and C are the predicted corresponding ventral clear patch (VCP) phenotypes visible by low magnification stereomicroscopy (asterisk marks partial VCP in panel C). Scale bar in panel A (for A-C) is 50 μm.

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