Fig 7.tif (464.32 kB)
Characterization of the scFvF7-Fc-vcMMAE conjugate.
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posted on 2018-02-08, 18:45 authored by Aleksandra Borek, Aleksandra Sokolowska-Wedzina, Grzegorz Chodaczek, Jacek Otlewski(a) SDS-PAGE analysis of scFvF7-Fc and scFvF7-Fc-vcMMAE under reducing conditions. (b-d) Cytotoxicity of scFvF7-Fc-vcMMAE, scFvF7-Fc and MMAE on (b) Snu-16, (c) NCI-H716, and (d) U2OS cells. Cell viability was determined by AlamarBlue Proliferation Assay Kit. Data show average values of triplicate wells ± SD from a representative experiment of two that were performed.
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FGFR 2-positive tumor cellsaffinityNCI-HscFvF 7-Fc antibodyMMAEfragment scFv phage display libraries Tomlinsonextracellular ligand-binding domaininternalizing anti-FGFR 2 single-chainECDcytotoxic drug monomethyl-auristatin EFGF receptor 2bivalent diabody formatFGFR 2cancers Fibroblast growth factor receptorsnanomolar K D valuesscFvF 7-FcFGFR 2 overexpressionphage display technology
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