Characterization of epitheloid cell sheet on APLM.

(A) Epitheloid cell sheets under airlifting culture. (B) HE staining of epitheloid cell sheet. (C-G) Immunofluorescent staining of ABCG2 (C), p63 (D), CK14 (E), CK3 (F), tubulin β3 (G), and CK1/10 (H) in the cells cultured on APLM. Scale bar, 20 μm. (I-J) Gene (I) and protein (J) expression of ABCG2, p63, CK14, and CK3 in epitheloid cell sheets. hESCs seeded on APLM were used as the control. Data are representative of three experiments and presented as the mean ± SD.*p < 0.05, **p < 0.01.