Changes in the level of the γ-AchR subunit.
A. Graph of the optical density (OD) of bands from western blots probed for the γ-AchR subunit (relative to β-actin) in SS compared to control muscle. The amount of the γ-AchR subunit protein, typically expressed in denervated and fetal muscle, was assessed in protein extracts prepared from homogenized SS and control deltoid muscle (* indicates significant difference, p<<0.001, N = 19 paired samples). B. A representative western blot prepared from protein extracts loaded into lanes for control (C) and Supraspinatus (SS) muscles from different participants, probed to detect the γ-AchR subunit and then re-probed to detect β-actin (as a loading control).