C2 induces mitochondrial mediated apoptosis in L.major promastigotes.
a) FACS analysis showing the reduction of mitochondrial potential and the induction of apoptosis in the C2NL treated L.major promastigotes. Promastigotes were treated with 30μM of C2NL for a period of 48hours. The image shown is a representative image of three independent experiments b) Percentage of cells showing an increase in the number of cells in the late apoptotic phase in the treated samples as well reduction in the number of live cells. Experiment was performed in triplicates. Significance is indicated in the figures as *p<0.01. Error bars represent the standard deviation of the mean.