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Breeding schedule and outcome of the generation of En1KO;Pitx3GFP/GFP animals.

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posted on 2017-08-11, 17:40 authored by Willemieke M. Kouwenhoven, Lars von Oerthel, Marten P. Smidt

(A) En1Het;Pitx3GFP/GFP animals were inter-crossed to generate a litter that includes three genotypes: En1WT;Pitx3GFP/GFP, En1Het;Pitx3GFP/GFP, and En1KO;Pitx3GFP/GFP. (B) The presence of double En1KO;Pitx3GFP/GFP animals occurred below (Mendelian) predicted chance level (12.7% versus 25%). (C) DAPI staining at E14.5 reveals that in En1WT;Pitx3GFP/GFP and En1Het;Pitx3GFP/GFP animals the cerebellar anlage is correctly developed (Cb), whilst it is absent in the double En1KO;Pitx3GFP/GFP (*). (Rf: retroflexus, Cb: cerebellum, Cp: choroid plexus).

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    ventral tegmental areaEn 1 tm 1AljEn 1 control3KOprogramming mdDA neuronshomeobox transcription factor Engrailed 1En 1KO animalsdopaminergic cell fatepool Mesodiencephalic dopaminergicEn 1VTAAhd 2 expressionmdDA neuronsPitx 3paired-like homeodomain transcription factor 3En 1 expressioncaudal subset identity

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