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Body cavity neurons modulate feeding behavior.

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posted on 2019-12-05, 19:01 authored by Aude D. Bouagnon, Lin Lin, Shubhi Srivastava, Chung-Chih Liu, Oishika Panda, Frank C. Schroeder, Supriya Srinivasan, Kaveh Ashrafi

(A) EGL-2 is expressed in a limited subset of sensory neurons. Epifluorescent image of a transgenic animal expressing egl-2p::gfp transcriptional reporter. Orange and green arrows indicate the AQR and URX body cavity neurons, respectively. (B) Animals expressing egl-2(gf) in body cavity neurons do not elevate feeding in response to 5mM 5HT. Relative pharyngeal pumping rates of wild-type and gcy-32p::egl-2(gf) expressing animals treated with vehicle or 5 mM 5HT. (C) Constitutive activation of synaptic release from body cavity neurons stimulates feeding. Relative pharyngeal pumping rates of wild-type, acox-1(ok2257), CX10386 gcy-32p::pkc-1(gf), and acox-1; CX10386 gcy-32p::pkc-1(gf) animals treated with vehicle or 5 mM 5HT. (D) Cell-specific RNAi of egl-2 in URX neurons is sufficient to rescue feeding defects of acox-1 mutants. Sense and antisense (SAS) transgenes targeting egl-2 were expressed under the control of flp-8, a URX specific promoter. Relative pharyngeal pumping rates of wild-type, acox-1(ok2257) and three independent lines of acox-1; flp-8p::egl-2(SAS) animals treated with vehicle or 5 mM 5HT. In (B) and (C), feeding data are normalized to vehicle-treated wild-type animals and are presented as a percentage of wild-type animals. Error bars indicate ±SEM from normalized mean, n = 15 animals per condition. ***p < 0.001 two-way ANOVA (Tukey). All feeding data are normalized to vehicle-treated wild-type animals and are presented as a percentage of wild-type rates. See S1 Table and S1 Data for underlying data. acox-1, acyl-CoA oxidase 1; EGL-2, EGg-Laying defective 2; flp-8, FMRF-Like Peptide 8; SAS, sense and antisense; WT, wild-type; 5HT, 5-hydroxytryptamine.

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