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Blocking the production of TH abrogates the beneficial effects of UA on thermogenesis and body weight control.

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posted on 2020-03-27, 17:34 authored by Bo Xia, Xiao Chen Shi, Bao Cai Xie, Meng Qing Zhu, Yan Chen, Xin Yi Chu, Guo He Cai, Min Liu, Shi Zhen Yang, Grant A. Mitchell, Wei Jun Pang, Jiang Wei Wu

(A) Schematic diagram of mice treatment. UA- and vehicle-treated mice were given PTU (8.5 mg/100 ml in the drinking water), an inhibitor of TH synthesis, for a period of 7-week HFD feeding. (B) Body weight time course (n = 6). (C) Daily food intake (n = 6). Weight of (D) total fat mass, (E) BAT, and (F) iWAT (n = 6). (G) GTT and (H) ITT performed in mice (n = 6). (I) Body temperature in mice placed at 21°C (n = 6). mRNA expression of thermogenic genes in (J) BAT and (K) iWAT (n = 6). Immunoblot of UCP-1 and PGC-1α protein in (L) BAT and (M) iWAT (n = 3). C+: positive control from BAT. The underlying data for this figure can be found in S1 Data. BAT, brown adipose tissue; Cidea, Cell death inducing DFFA like effector a; Cox7a1, Cytochrome c oxidase subunit 7a1; Dio2, Deiodinase 2; GTT, glucose tolerance test; HFD, high-fat diet; ITT, insulin tolerance test; iWAT, inguinal white adipose tissue; Pgc1-α, Peroxisome proliferator-activated receptor gamma coactivator 1α; PTU, propylthiouracil; TH, thyroid hormone; UA, urolithin A; UCP-1, Uncoupling protein 1.

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