Assessment of ‘DNA-breaks’ in nuclei and gaps in kinetoplast circles.
2018-11-26T19:48:08Z (GMT) by
<p>(<b>A</b>) Percentage of cells positive for a TUNEL signal. ***, <i>P</i> < 0.001 from Students <i>t</i>-test. The microscopy images show an example of the kinetoplast-associated TUNEL signal in NT cells. (<b>B</b>) Example images of TUNEL signals, always associated with the kinetoplast (k) rather than the nucleus (n), at different cell cycle stages; G<sub>1</sub>, S, G<sub>2</sub> and post M (post mitotic). Dashed boxes indicate magnified regions shown in C. (<b>C</b>) Percentage of cells in each cycle stage positive for TUNEL signals, inset images show magnified signals from B as indicated by dashed boxes. (<b>D</b>) Examples of TUNEL signals in none or all four kinetoplasts following suramin treatment. (<b>E</b>) Percentage of cells positive for γH2A signals. The microscopy images show an example of the nuclear γH2A signal in NT cells. MMS, methyl methanesulfonate. Other details in A, C and E as in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006980#pntd.0006980.g002" target="_blank">Fig 2A</a>; error bars, SD; N = 2; all scale bars, 5 μm.</p>