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Analysis of membrane permeability, cell morphology and antigen expression in TransFix® treated cells.

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posted on 2016-09-07, 17:35 authored by Cecilia González, Rosa Esteban, Carme Canals, Eduardo Muñiz-Díaz, Núria Nogués

A. Staining with 7-AAD reagent at different times after treatment and comparison with equivalent fresh cells. B. Forward (FSC) and side scatter (SSC) parameters were acquired at several time points (shown for 120 days), and compared with fresh cells. C. Antigen expression levels of MELDia cells (HIRO.71 staining) or CHOLua cells (BRIC221 staining) at 120 days of treatment (fresh cells are overlaid); negative controls were MELwt or CHOwt cells stained in matching conditions.

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    transfected cells antigenic propertieslow-incidence blood group antigensblood group antigensmembrane permeabilitycell linescell preservation methodsantigen expression levelsLow-Incidence Blood Group AntigensNovel Methods Facilitatinglow-incidence blood group phenotypescell stabilization methodsRBCcell culture facilities

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