Analysis of membrane permeability, cell morphology and antigen expression in TransFix® treated cells.
A. Staining with 7-AAD reagent at different times after treatment and comparison with equivalent fresh cells. B. Forward (FSC) and side scatter (SSC) parameters were acquired at several time points (shown for 120 days), and compared with fresh cells. C. Antigen expression levels of MELDia cells (HIRO.71 staining) or CHOLua cells (BRIC221 staining) at 120 days of treatment (fresh cells are overlaid); negative controls were MELwt or CHOwt cells stained in matching conditions.