Analyses of plc1 and plc2 transcription in ATCC 19606^T and isogenic derivatives.

(A) Transcriptional analyses of plc1 and plc2 in ATCC 19606^T cells grown in TSBD or TSBD supplemented with 50 μM FeCl₃ (TSBD + Fe). Expression of bauA was used as a positive control for iron-regulated gene expression. (B) Transcriptional analyses of plc1 and plc2 genes to determine any compensatory regulation in cells of the ATCC 19606^T parental strain or the isogenic derivatives 3430 (plc2::aph) or 3452 (plc1::aph-FRT) cells grown in TSBD for 24 h at 37°C with shaking at 200 rpm. Expression of plc genes was normalized to the expression of the 16S gene, which is constitutively expressed under iron-rich and iron-chelated conditions. Error bars represent the standard error (SE) of the mean.