Agarose gel electrophoresis of PCR products using primers located throughout the coding region and its 5’ upstream of Nc2^o-ADH.

(A) shows the location and direction of the PCR primers. The thick bar is the NC2^o-ADH coding region and the blue shows the protospacer site. The scale is 100 bp long. (B-D) are agarose gel images of PCR products. The templates used were genomic DNA isolated from clone 1-p-11, knockout mutants 1-3-17 and 2-3-52. Primer pairs used are indicated on top of each lane.