Adipose tissue inflammation is slightly increased in macrophage CD40 deficient mice without resulting in altered cytokine release.

(A) Quantification of CD45 staining for leukocytes of EpAT (n = 16 for HFD and n = 7 for SFD). (B) Quantification of Mac3 staining for macrophages in EpAT (n = 16 for HFD and n = 7 for SFD). (C) Quantification of CD3⁺ staining for T cells in EpAT (n = 16 for HFD and n = 7 for SFD). (D) Flow cytometric analysis of leukocyte subsets in EpAT, as % of total CD45⁺ leukocytes (n = 7-9/group). (E) mRNA expression of inflammatory markers in EpAT (n = 7-9/group). Data is presented as mean ± SEM.