Activin A in vaccinia infection of mice.
Activin A was detected by ELISA in tissue homogenates of primary vaccinia lesions from (A) flaky tail mice infected with ACAM-2000, and (B) retain lesional tissue from SCID mice infected with vaccinia strains IHDJ and Dryvax. (C-E) ACAM-2000 infection was carried out in CTX treated FT mice as described in Fig 2. The TGFβR inhibitor SB431542, or vehicle control, was applied topically beginning on day 1 post infection. On day 20, primary lesion skin (D) and ovary tissue (E) were collected and viral burden was analyzed by plaque assay. Representative data from 2 experiments are shown. Asterisk, p≤0.05, or as noted in figure.