A cellular epithelial-specific enhancer is amplified in the 20861 super-enhancer.
A, The strong cellular H3K27ac peak shown in Fig 5A aligned with an epithelial-specific enhancer (boxed region) identified from ENCODE. The data from the seven cell lines are represented as colored peaks in the layered H3K27ac track and are highlighted according to the ChIP-seq profiles from the ENCODE/Broad Institute track. The amplified region is marked by a black horizontal bar; the HPV16 genome insertion point is marked by a green arrowhead. B, The H3K27ac ChIP-seq profiles from individual cell types are shown (GM12878, B-lymphocytes; H1-hESC, human embryonic stem cells; K562, human erythroleukemic cell line; HeLa-S3, cervical carcinoma; HepG2, hepatocellular carcinoma; HUVEC, human umbilical vein cells; CD14+, CD14-positive monocytes; HMEC, human mammary epithelial cells; HSMM, human skeletal muscle myoblasts; NH-A, normal human astrocytes; NHDF-Ad, normal human dermal fibroblasts-adult; NHEK, normal human epidermal keratinocytes; NHLF, normal human lung fibroblasts; MCF-7, mammary gland adenocarcinoma; HCT-116, colorectal carcinoma; PANC-1, pancreatic carcinoma). C, H2A.Z, H3K9ac, H3K4me1, H3K4me2, and H3K27ac tracks in NHEK cells from the ENCODE/Broad Institute track.