A Teleost Bactericidal Permeability-Increasing Protein Kills Gram-Negative Bacteria, Modulates Innate Immune Response, and Enhances Resistance against Bacterial and Viral Infection - Fig 4

2016-04-22T06:17:21Z (GMT) by Yuan-yuan Sun Li Sun
<p><b>Binding of rCsBPI to bacteria detected by ELISA (A) and microscopy (B).</b> (A) <i>Edwardsiella tarda</i>, <i>Pseudomonas</i> <i>f</i><i>luorescens</i>, <i>Vibrio anguillarum</i>, <i>Vibrio harveyi</i>, <i>Streptococcus iniae</i>, <i>Micrococcus luteus</i>, and <i>Staphylococcus aureus</i> were incubated with or without (control) different concentrations of rCsBPI, and binding of the protein to bacterial cells was determined by ELISA. Values are shown as means ± SEM (N = 3). N, the number of times the experiment was performed. (B) <i>P</i>. <i>fluorescens</i> was incubated with rCsBPI (B1 and B3) or rTrx (B2 and B4), and the cells were treated with anti-His antibody and FITC-labeled secondary antibody. The cells were then examined under a fluorescence microscope with (B3 and B4) or without (B1 and B2) fluorescence light. Bar: 4 μm.</p>