A Teleost Bactericidal Permeability-Increasing Protein Kills Gram-Negative Bacteria, Modulates Innate Immune Response, and Enhances Resistance against Bacterial and Viral Infection - Fig 4
Binding of rCsBPI to bacteria detected by ELISA (A) and microscopy (B). (A) Edwardsiella tarda, Pseudomonas fluorescens, Vibrio anguillarum, Vibrio harveyi, Streptococcus iniae, Micrococcus luteus, and Staphylococcus aureus were incubated with or without (control) different concentrations of rCsBPI, and binding of the protein to bacterial cells was determined by ELISA. Values are shown as means ± SEM (N = 3). N, the number of times the experiment was performed. (B) P. fluorescens was incubated with rCsBPI (B1 and B3) or rTrx (B2 and B4), and the cells were treated with anti-His antibody and FITC-labeled secondary antibody. The cells were then examined under a fluorescence microscope with (B3 and B4) or without (B1 and B2) fluorescence light. Bar: 4 μm.