Fig 7.tif (796.84 kB)
ARE reporter response in mesenchymal type cells.
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posted on 2017-06-01, 17:34 authored by Waqas Azeem, Margrete Reime Hellem, Jan Roger Olsen, Yaping Hua, Kristo Marvyin, Yi Qu, Biaoyang Lin, Xisong Ke, Anne Margrete Øyan, Karl-Henning Kalland(A) FACS analysis of mCherry signals in EPT3-PT1-AR-241B cells. Cells were grown in androgen free medium and treated with different AR agonists (1 nM R1881 and 1 μM DHT), steroids (10 nM dexamethasone, 10 nM progesterone and 1 μM β-estradiol), AR antagonist (10 μM enzalutamide) and anti-androgen (10 μM abiraterone). (B) Texas red (Tx-Red) indirect immunofluorescent detection of AR in EPT3-PT1-AR-241B cells. The cells were treated with ± 1 nM R1881 for 24 hours.
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cell lines transducedluciferase reporter genesreporter activityLNCaP cellsluminal differentiation transcriptometumorigenic mesenchymal progeny cellsandrogen synthesis inhibitorlentiviral vector backbonesepithelial cell linesprostate cellsreporter vectorsAR target gene activationbackground reporter activitymesenchymal type cellsprostate cancerCRPCprostate cancer cellsandrogen receptor activityEP 156T cellsandrogen response element
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