Subcellular localization analyses of PmCFAT1 and PmCFAT2. ZhangTengxun HuoTingting DingAnqi HaoRuijie WangJia ChengTangren BaoFei ZhangQixiang 2019 <p>(A) Prediction of protein subcellular localization of PmCFATs with WoLF PSORT Server. Note: nucl represents nucleus; cyto represents cytoplasm; mito represents mitochondria; plas represents plasma membrane; chlo represents chloroplast. (B) Restriction enzyme digestion of <i>pSuper1300-GFP-PmCFATs</i>. M: DL 15,000 Marker; 1 and 3 represent the enzyme digestion products of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively; 2 and 4 represent circular plasmids of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively, as controls. (C) Subcellular localization of PmCFAT1 and PmCFAT2. The fusion proteins were observed under a confocal laser scanning microscope. a, e, i show the green fluorescence channel; b, f, j show the chloroplast autofluorescence channel. c, g and k show the bright field channel; d, h and l were created from the images shown in the first two panels. a-d: bar = 10 μm; e-l: bar = 15 μm.</p>