10.1371/journal.pone.0223974.g006 Tengxun Zhang Tengxun Zhang Tingting Huo Tingting Huo Anqi Ding Anqi Ding Ruijie Hao Ruijie Hao Jia Wang Jia Wang Tangren Cheng Tangren Cheng Fei Bao Fei Bao Qixiang Zhang Qixiang Zhang Subcellular localization analyses of PmCFAT1 and PmCFAT2. Public Library of Science 2019 coniferyl alcohol acetyltransferase genes enzyme activity assays Prunus mume Prunus mume enzyme activity analysis Coniferyl alcohol acetyltransferase 2 PmCFAT genes eugenol biosynthesis GC-MS DFGFG expression DFGWG Subcellular localization analysis eugenol synthesis BAHD acyltransferase family PmBAHD 67-70 proteins PmCFAT 1 PmCFAT 2 90 PmBAHD genes 2019-10-16 20:07:12 Figure https://plos.figshare.com/articles/figure/Subcellular_localization_analyses_of_PmCFAT1_and_PmCFAT2_/9992249 <p>(A) Prediction of protein subcellular localization of PmCFATs with WoLF PSORT Server. Note: nucl represents nucleus; cyto represents cytoplasm; mito represents mitochondria; plas represents plasma membrane; chlo represents chloroplast. (B) Restriction enzyme digestion of <i>pSuper1300-GFP-PmCFATs</i>. M: DL 15,000 Marker; 1 and 3 represent the enzyme digestion products of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively; 2 and 4 represent circular plasmids of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively, as controls. (C) Subcellular localization of PmCFAT1 and PmCFAT2. The fusion proteins were observed under a confocal laser scanning microscope. a, e, i show the green fluorescence channel; b, f, j show the chloroplast autofluorescence channel. c, g and k show the bright field channel; d, h and l were created from the images shown in the first two panels. a-d: bar = 10 μm; e-l: bar = 15 μm.</p>