10.1371/journal.pone.0223974.g006
Tengxun Zhang
Tengxun
Zhang
Tingting Huo
Tingting
Huo
Anqi Ding
Anqi
Ding
Ruijie Hao
Ruijie
Hao
Jia Wang
Jia
Wang
Tangren Cheng
Tangren
Cheng
Fei Bao
Fei
Bao
Qixiang Zhang
Qixiang
Zhang
Subcellular localization analyses of PmCFAT1 and PmCFAT2.
Public Library of Science
2019
coniferyl alcohol acetyltransferase genes
enzyme activity assays
Prunus mume Prunus mume
enzyme activity analysis
Coniferyl alcohol acetyltransferase
2 PmCFAT genes
eugenol biosynthesis
GC-MS
DFGFG
expression
DFGWG
Subcellular localization analysis
eugenol synthesis
BAHD acyltransferase family
PmBAHD 67-70 proteins
PmCFAT 1
PmCFAT 2
90 PmBAHD genes
2019-10-16 20:07:12
Figure
https://plos.figshare.com/articles/figure/Subcellular_localization_analyses_of_PmCFAT1_and_PmCFAT2_/9992249
<p>(A) Prediction of protein subcellular localization of PmCFATs with WoLF PSORT Server. Note: nucl represents nucleus; cyto represents cytoplasm; mito represents mitochondria; plas represents plasma membrane; chlo represents chloroplast. (B) Restriction enzyme digestion of <i>pSuper1300-GFP-PmCFATs</i>. M: DL 15,000 Marker; 1 and 3 represent the enzyme digestion products of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively; 2 and 4 represent circular plasmids of <i>pSuper1300</i>::<i>PmCFAT1</i>::<i>GFP</i> and <i>pSuper1300</i>::<i>PmCFAT2</i>::<i>GFP</i>, respectively, as controls. (C) Subcellular localization of PmCFAT1 and PmCFAT2. The fusion proteins were observed under a confocal laser scanning microscope. a, e, i show the green fluorescence channel; b, f, j show the chloroplast autofluorescence channel. c, g and k show the bright field channel; d, h and l were created from the images shown in the first two panels. a-d: bar = 10 μm; e-l: bar = 15 μm.</p>