Ca<sup>2+</sup> dependence of proPO-casp release and the inhibitory effect of a caspase-1 inhibitor and caspase-1 knockdown <i>in vitro</i>. Miti Jearaphunt Chadanat Noonin Pikul Jiravanichpaisal Seiko Nakamura Anchalee Tassanakajon Irene Söderhäll Kenneth Söderhäll 10.1371/journal.ppat.1004059.g003 https://plos.figshare.com/articles/figure/_Ca_2_dependence_of_proPO_casp_release_and_the_inhibitory_effect_of_a_caspase_1_inhibitor_and_caspase_1_knockdown_in_vitro_/995360 <p>Granular cells (GC) were incubated in buffer containing different concentrations of Ca<sup>2+</sup>. The presence of proPO-casps outside the cells was examined in medium at 30 and 60 min. The release of proPO-casps was Ca<sup>2+</sup> and time dependent (A) and was inhibited when the cells were preincubated for 30 min with the caspase-1 inhibitor Z-YVAD-FMK prior to Ca<sup>2+</sup> addition (B). The caspase-1-like transcript was completely decreased after 65 h of caspase-1 dsRNA (dsCaspase-1) treatment when compared to the dsGFP control (C). The level of proPO-casp fragments were decreased in both cell lysate and medium in dsCaspase-1 treated group when compared to dsGFP (D).</p> 2014-04-10 04:07:25 Evolutionary biology Evolutionary immunology immunology Immune response Immune system dependence propo-casp inhibitory caspase-1 inhibitor knockdown