Thuy Duong, Nguyen Morris, Glenn E. Lam, Le Thanh Zhang, Qiuping A. Sewry, Caroline M. Shanahan, Catherine Holt, Ian Tissue-dependent alternative splicing of the highly conserved DV23 exon of nesprin-1 and nesprin-2 and the KASH domain of nesprin-2. <p>Products of conventional PCR were separated on agarose gels. The 27 human cDNA samples, as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094380#pone-0094380-t001" target="_blank">Table 1</a>, were used in A and B. For DV23, the upper band contained the DV23 region and the lower band lacked the DV23 region. Specific primer pairs spanned: (A) The nesprin-1 DV23 region of nesprin-1-giant and other nesprin-1 isoforms, (B) the nesprin-2 DV23 region of nesprin-2-giant (which included other nesprin-2 isoforms except nesprin-2-alpha-1) and (C) the nesprin-2 DV23 region of only the nesprin-2-alpha-1 isoform. Vertical black lines indicate images from different gels that have been compiled. (D) PCR was used to detect the presence of nesprin-2 KASH, along with GAPDH control in 5 cDNA samples.</p> cell biology;Cellular structures and organelles;Cell membranes;Membrane proteins;Transmembrane proteins;Molecular cell biology;Biochemistry;dna;DNA amplification;proteins;Structural proteins;rna;RNA synthesis;Nucleic acids;immunology;immunochemistry;genetics;gene expression;Gene regulation;Genetic dominance;Autosomal dominant traits;Human genetics;Molecular genetics;cardiology;Cardiovascular diseases;cardiomyopathies;neurology;Muscular dystrophies;splicing;conserved;dv23;exon;nesprin-1;nesprin-2;kash 2014-04-09
    https://plos.figshare.com/articles/figure/_Tissue_dependent_alternative_splicing_of_the_highly_conserved_DV23_exon_of_nesprin_1_and_nesprin_2_and_the_KASH_domain_of_nesprin_2_/993477
10.1371/journal.pone.0094380.g007