Evidence at the protein level for nesprin-2-epsilon-1 in Ntera-2 and ESC cells and for nesprin-2-epsilon-2 in heart and brain.
Nguyen Thuy Duong
Glenn E. Morris
Le Thanh Lam
Qiuping Zhang
Caroline A. Sewry
Catherine M. Shanahan
Ian Holt
10.1371/journal.pone.0094380.g005
https://plos.figshare.com/articles/figure/_Evidence_at_the_protein_level_for_nesprin_2_epsilon_1_in_Ntera_2_and_ESC_cells_and_for_nesprin_2_epsilon_2_in_heart_and_brain_/993473
<p>Western blot for nesprin-2 in human tissues and cell lines using antibody against the C-terminal region of nesprin-2 (MANNES2A 11A3). Bands of approximate size of nesprin-2-epsilon-1 (122 kD) were detected in Ntera-2 and ESC and bands the size of nesprin-2-epsilon-2 (103 kD) were detected in brain and heart. Nesprin-2-alpha-1 was observed in skeletal muscle, but epsilon isoforms were not detected. Absence of mRNA (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094380#pone.0094380.s001" target="_blank">Table S4 in File S1</a>), indicates that putative nesprin-2-gamma (377 kD) and nesprin-2-alpha-2 (47 kD) bands on the skeletal muscle western blot, are likely to be degradation products.</p>
2014-04-09 03:41:15
cell biology
Cellular structures and organelles
Cell membranes
Membrane proteins
Transmembrane proteins
Molecular cell biology
Biochemistry
dna
DNA amplification
proteins
Structural proteins
rna
RNA synthesis
Nucleic acids
immunology
immunochemistry
genetics
gene expression
Gene regulation
Genetic dominance
Autosomal dominant traits
Human genetics
Molecular genetics
cardiology
Cardiovascular diseases
cardiomyopathies
neurology
Muscular dystrophies
nesprin-2-epsilon-1
ntera-2
esc
cells
nesprin-2-epsilon-2