Guðmundsdóttir, Ragnhildur Kreiling, Agnes-Katharina Kristjánsson, Bjarni Kristófer Marteinsson, Viggó Þór Pálsson, Snæbjörn Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod <i>Crangonyx islandicus</i> <div><p><i>Crangonyx islandicus</i> is a groundwater amphipod endemic to Iceland, considered to have survived the Ice Ages in subglacial refugia. Currently the species is found in spring sources in lava fields along the tectonic plate boundary of the country. The discovery of a groundwater species in this inaccessible habitat indicates a hidden ecosystem possibly based on chemoautotrophic microorganisms as primary producers. To explore this spring ecosystem, we assessed its microbial diversity and analysed whether and how the diversity varied between the amphipods and the spring water, and if was dependent on environmental factors and geological settings. Isolated DNA from spring water and from amphipods was analysed using metabarcoding methods, targeting the 16S rRNA gene. Two genera of bacteria, <i>Halomonas</i> and <i>Shewanella</i> were dominating in the amphipod samples in terms of relative abundance, but not in the groundwater samples where <i>Flavobacterium</i>, <i>Pseudomonas</i> and <i>Alkanindiges</i> among others were dominating. The richness of the bacteria taxa in the microbial community of the groundwater spring sources was shaped by pH level and the beta diversity was shaped by geographic locations.</p></div> DNA;diversity;groundwater spring sources;tectonic plate boundary;16 S rRNA gene;spring water;spring sources;groundwater amphipod Crangonyx islandicus Crangonyx islandicus 2019-10-02
    https://plos.figshare.com/articles/dataset/Bacterial_diversity_in_Icelandic_cold_spring_sources_and_in_relation_to_the_groundwater_amphipod_i_Crangonyx_islandicus_i_/9930248
10.1371/journal.pone.0222527