10.1371/journal.pone.0093123.g006 Dagmar Fichtner Dagmar Fichtner Bärbel Lorenz Bärbel Lorenz Sinem Engin Sinem Engin Christina Deichmann Christina Deichmann Marieelen Oelkers Marieelen Oelkers Andreas Janshoff Andreas Janshoff Andre Menke Andre Menke Doris Wedlich Doris Wedlich Clemens M. Franz Clemens M. Franz Dynamic force spectroscopy. Public Library of Science 2014 Biochemistry proteins Protein interactions Recombinant proteins Biomacromolecule-ligand interactions chemical biology cytochemistry biophysics biotechnology Bioengineering Biological systems engineering Biomedical Engineering Biomimetics biomaterials Bionanotechnology cell biology cell adhesion cadherins Cellular structures and organelles Cell membranes Membrane proteins Transmembrane proteins Molecular cell biology chemistry 2014-03-27 03:55:26 Figure https://plos.figshare.com/articles/figure/_Dynamic_force_spectroscopy_/977378 <p>(A) Rupture force distributions obtained at different retraction speeds for the homotypic E1-5 bond rupture. (B) Rupture force <i>F</i><sub>f</sub> as a function of loading rate for homotypic E1-5 (green symbols) or heteromeric E1-2/E1-5 bond rupture (orange symbols). The different symbols represent the maxima identified in the multimodal distribution of rupture force obtained for a given loading rate. For the homotypic E1-5 bond we determined x<sub>u</sub>  =  1.1 nm and k<sub>off</sub>  =  1.2 × 10<sup>−4</sup> s<sup>−1</sup> (green circles); x<sub>u</sub>  =  2.3 nm and k<sub>off</sub>  =  1.4 × 10<sup>−5</sup> s<sup>−1</sup> (green triangles). For the heteromeric E1-5/E1-2 bond we determined x<sub>u</sub>  =  4.5 nm and k<sub>off</sub>  =  5.9 × 10<sup>−7</sup> s<sup>−1</sup> (yellow triangles). The violet stars indicate data taken from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093123#pone.0093123-Bayas1" target="_blank">[41]</a>.</p>