%0 Figure %A Yamashita, Hironori %A Kotani, Takenori %A Park, Jung-ha %A Murata, Yoji %A Okazawa, Hideki %A Ohnishi, Hiroshi %A Ku, Yonson %A Matozaki, Takashi %D 2014 %T Generation of IEC-specific Shp2 CKO mice. %U https://plos.figshare.com/articles/figure/_Generation_of_IEC_specific_Shp2_CKO_mice_/977301 %R 10.1371/journal.pone.0092904.g001 %2 https://plos.figshare.com/ndownloader/files/1438899 %K anatomy %K biological tissue %K epithelium %K Epithelial cells %K cell biology %K Cell processes %K Cell growth %K Signal transduction %K cell signaling %K Signaling cascades %K Tyrosine kinase signaling cascade %K Cell movement signaling %K Ras signaling %K Cellular types %K Molecular cell biology %K Gastroenterology and hepatology %K Inflammatory bowel disease %K Model organisms %K Animal models %K Mouse models %K iec-specific %K shp2 %K cko %X

A: Genomic DNA extracted from the indicated organs of adult control (Ctrl) or Shp2 CKO (CKO) mice was subjected to PCR analysis with primers specific for deleted or floxed alleles of Ptpn11. Data are representative of three separate experiments. B: Lysates of IECs (from the ileum or colon) and the indicated organs from control or Shp2 CKO mice were subjected to immunoblot analysis with antibodies to (α-) both Shp2 and β-tubulin (loading control). Data are representative of three (IECs) or two (indicated organs) separate experiments.

%I PLOS ONE