Peano, Clelia Chiaramonte, Fabrizio Motta, Sara Pietrelli, Alessandro Jaillon, Sebastien Rossi, Elio Consolandi, Clarissa L. Champion, Olivia L. Michell, Stephen Freddi, Luca Falciola, Luigi Basilico, Fabrizio Garlanda, Cecilia Mauri, Pierluigi De Bellis, Gianluca Landini, Paolo Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I. <p>Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from <i>E. coli</i> Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.</p> Biochemistry;rna;RNA stability;proteomics;Computational biology;genome analysis;Transcriptome analysis;Genome expression analysis;genetics;gene expression;Gene regulation;genomics;Functional genomics;Molecular genetics;microbiology;Microbial physiology;molecular biology;Molecular biology techniques;Sequencing techniques;Genome sequencing;Infectious diseases;Emerging infectious diseases;Pathology and laboratory medicine;pathogenesis;Host-pathogen interactions;macrophage;exposed;btcdc272;grown 2014-03-26
    https://plos.figshare.com/articles/figure/_Cytokine_production_by_mouse_macrophage_cell_line_RAW264_7_exposed_to_BtCDC272_grown_in_different_conditions_and_at_different_M_O_I_/975187
10.1371/journal.pone.0093009.g008