Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I. PeanoClelia ChiaramonteFabrizio MottaSara PietrelliAlessandro JaillonSebastien RossiElio ConsolandiClarissa L. ChampionOlivia L. MichellStephen FreddiLuca FalciolaLuigi BasilicoFabrizio GarlandaCecilia MauriPierluigi De BellisGianluca LandiniPaolo 2014 <p>Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from <i>E. coli</i> Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.</p>