%0 Figure %A Peano, Clelia %A Chiaramonte, Fabrizio %A Motta, Sara %A Pietrelli, Alessandro %A Jaillon, Sebastien %A Rossi, Elio %A Consolandi, Clarissa %A L. Champion, Olivia %A L. Michell, Stephen %A Freddi, Luca %A Falciola, Luigi %A Basilico, Fabrizio %A Garlanda, Cecilia %A Mauri, Pierluigi %A De Bellis, Gianluca %A Landini, Paolo %D 2014 %T Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I. %U https://plos.figshare.com/articles/figure/_Cytokine_production_by_mouse_macrophage_cell_line_RAW264_7_exposed_to_BtCDC272_grown_in_different_conditions_and_at_different_M_O_I_/975187 %R 10.1371/journal.pone.0093009.g008 %2 https://plos.figshare.com/ndownloader/files/1436231 %K Biochemistry %K rna %K RNA stability %K proteomics %K Computational biology %K genome analysis %K Transcriptome analysis %K Genome expression analysis %K genetics %K gene expression %K Gene regulation %K genomics %K Functional genomics %K Molecular genetics %K microbiology %K Microbial physiology %K molecular biology %K Molecular biology techniques %K Sequencing techniques %K Genome sequencing %K Infectious diseases %K Emerging infectious diseases %K Pathology and laboratory medicine %K pathogenesis %K Host-pathogen interactions %K macrophage %K exposed %K btcdc272 %K grown %X

Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from E. coli Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.

%I PLOS ONE