Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I.
Clelia Peano
Fabrizio Chiaramonte
Sara Motta
Alessandro Pietrelli
Sebastien Jaillon
Elio Rossi
Clarissa Consolandi
Olivia L. Champion
Stephen L. Michell
Luca Freddi
Luigi Falciola
Fabrizio Basilico
Cecilia Garlanda
Pierluigi Mauri
Gianluca De Bellis
Paolo Landini
10.1371/journal.pone.0093009.g008
https://plos.figshare.com/articles/figure/_Cytokine_production_by_mouse_macrophage_cell_line_RAW264_7_exposed_to_BtCDC272_grown_in_different_conditions_and_at_different_M_O_I_/975187
<p>Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from <i>E. coli</i> Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.</p>
2014-03-26 03:02:27
Biochemistry
rna
RNA stability
proteomics
Computational biology
genome analysis
Transcriptome analysis
Genome expression analysis
genetics
gene expression
Gene regulation
genomics
Functional genomics
Molecular genetics
microbiology
Microbial physiology
molecular biology
Molecular biology techniques
Sequencing techniques
Genome sequencing
Infectious diseases
Emerging infectious diseases
Pathology and laboratory medicine
pathogenesis
Host-pathogen interactions
macrophage
exposed
btcdc272
grown