Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I. Clelia Peano Fabrizio Chiaramonte Sara Motta Alessandro Pietrelli Sebastien Jaillon Elio Rossi Clarissa Consolandi Olivia L. Champion Stephen L. Michell Luca Freddi Luigi Falciola Fabrizio Basilico Cecilia Garlanda Pierluigi Mauri Gianluca De Bellis Paolo Landini 10.1371/journal.pone.0093009.g008 https://plos.figshare.com/articles/figure/_Cytokine_production_by_mouse_macrophage_cell_line_RAW264_7_exposed_to_BtCDC272_grown_in_different_conditions_and_at_different_M_O_I_/975187 <p>Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from <i>E. coli</i> Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.</p> 2014-03-26 03:02:27 Biochemistry rna RNA stability proteomics Computational biology genome analysis Transcriptome analysis Genome expression analysis genetics gene expression Gene regulation genomics Functional genomics Molecular genetics microbiology Microbial physiology molecular biology Molecular biology techniques Sequencing techniques Genome sequencing Infectious diseases Emerging infectious diseases Pathology and laboratory medicine pathogenesis Host-pathogen interactions macrophage exposed btcdc272 grown