Effects of telomere end-to-end fusion and telomere tethering to the nuclear envelope on GCR formation. Hiroyuki Irie Io Yamamoto Yusuke Tarumoto Sanki Tashiro Kurt W. Runge Fuyuki Ishikawa 10.1371/journal.pgen.1008335.g003 https://plos.figshare.com/articles/figure/Effects_of_telomere_end-to-end_fusion_and_telomere_tethering_to_the_nuclear_envelope_on_GCR_formation_/9737993 <p>(A) GCR rates of wild-type, <i>taz1</i>Δ, and <i>rap1</i>Δ strains in the presence or absence of <i>lig4</i><sup>+</sup>. (B) GCR rates of strains with INM protein mutants. GFP-Bqt4ΔN or Rap1-GFP-Bqt4ΔN fusion protein was expressed from the <i>bqt4</i> promoter integrated into genomic DNA as described previously [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008335#pgen.1008335.ref023" target="_blank">23</a>]. (C) GCR rates of wild-type, <i>taz1</i>Δ, and <i>rap1</i>Δ strains in the presence or absence of <i>bqt4</i><sup>+</sup>. Asterisks represent significant differences (P < 0.05) from the wild-type strain unless otherwise noted. P-values were obtained by the two-tailed Mann–Whitney test.</p> 2019-08-27 17:37:35 GCR rates RAP 1 homologues chromosomal rearrangements fission yeast Genomic rearrangements telomere length homeostasis shelterin components Taz 1 I-SceI-mediated DSB-induction experiments TRF Telomere-binding proteins Taz 1 Poz 1-binding domain cause cell death chromosome fission yeast cells shelterin-mediated telomere functions Rap 1 DSB repair