%0 Figure %A Khater, Ismail M. %A Aroca-Ouellette, Stephane T. %A Meng, Fanrui %A Nabi, Ivan Robert %A Hamarneh, Ghassan %D 2019 %T The process of obtaining the class labels for the Cav1 blobs using wide-field CAVIN1/PTRF mask. The class labels are necessary to train the machine learning models to identify the Cav1 blobs types automatically. %U https://plos.figshare.com/articles/figure/The_process_of_obtaining_the_class_labels_for_the_Cav1_blobs_using_wide-field_CAVIN1_PTRF_mask_The_class_labels_are_necessary_to_train_the_machine_learning_models_to_identify_the_Cav1_blobs_types_automatically_/9731924 %R 10.1371/journal.pone.0211659.g002 %2 https://plos.figshare.com/ndownloader/files/17428799 %K classification %K Caveolae %K transcript release factor %K molecule localization microscopy %K 3 D cluster %K PC 3-PTRF cells %K adaptor protein polymerase %K PC 3 prostate cancer cells %K scaffold %K approach %K method %K caveolae %K point clouds %K 10 PC 3 %K accuracy %K Cav 1 proteins %K plasma membrane invaginations %K PointNet model %K SMLM data %K CAVIN %K CNN %K super-resolution microscopy images %K molecule localization microscopy data %X

(A) The first row shows the imaged wide-field TIRF CAVIN1/PTRF mask before and after morphological closing. The morphological closing operation is used to close the small holes in the consecutive regions of CAVIN1/PTRF mask. The CAVIN1/PTRF regions are delineated in yellow to highlight the locations of the CAVIN1/PTRF regions in the cell. (B) The second row shows the Cav1 blobs and the overlay of the Cav1 blobs with the wide-field CAVIN1/PTRF mask to label the blobs into PTRF+ and PTRF-. The caveolae structures have a minimum of 60 Cav1 molecule per blob [9] that can be used to stratify the PTRF+ blobs into PTRF+≥ 60 and PTRF+< 60. Our goal is to use machine learning approaches to automatically identify the PTRF+≥ 60 blobs (caveolar domains) from the rest of the non-caveolar domains (i.e. PTRF+< 60 and PTRF-) using different features and data representations of the blobs.

%I PLOS ONE