10.1371/journal.pone.0090473.t001 Min-Nan Tseng Min-Nan Tseng Chia-Ling Chung Chia-Ling Chung Shean-Shong Tzean Shean-Shong Tzean Primers and probes for real-time polymerase chain reaction (PCR) and reaction conditions. Public Library of Science 2014 agriculture Pest control biotechnology Bioengineering Genetic engineering Computational biology genome analysis Transcriptome analysis genetics genomics microbiology Medical microbiology Microbial pathogens Microbial control Mycology organisms fungi Pathology and laboratory medicine pathogens Virulence factors probes Real-time polymerase 2014-03-24 03:12:08 Dataset https://plos.figshare.com/articles/dataset/_Primers_and_probes_for_real_time_polymerase_chain_reaction_PCR_and_reaction_conditions_/972104 <p>*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C for 1 min, and extension at 72°C for 2 min, 30 cycles; elongation at 72°C for 5 min, 1 cycle.</p>