10.1371/journal.pone.0220894.g005 Leonore Wigger Leonore Wigger Cristina Casals-Casas Cristina Casals-Casas Michaël Baruchet Michaël Baruchet Khanh B. Trang Khanh B. Trang Sylvain Pradervand Sylvain Pradervand Aurélien Naldi Aurélien Naldi Béatrice Desvergne Béatrice Desvergne System analysis of cross-talk between nuclear receptors reveals an opposite regulation of the cell cycle by LXR and FXR in human HepaRG liver cells - Fig 5 Public Library of Science 2019 GW 3965 GW 4064 Global gene expression LXR pro-proliferative effect NR activities target genes liver cells HepaRG liver cells Transcriptional regulations 4 hours agonist glucose metabolism system analysis 24 hours receptor outcome NR specificity FXR ligand cell line HepaRG CDCA hepatocyte differentiation PPAR hepatocyte cells division HepaRG cells cell cycle cell cycle progression 2019-08-22 17:38:53 Figure https://plos.figshare.com/articles/figure/System_analysis_of_cross-talk_between_nuclear_receptors_reveals_an_opposite_regulation_of_the_cell_cycle_by_LXR_and_FXR_in_human_HepaRG_liver_cells_-_Fig_5/9720371 <p><b>Validation of the effects of FXR and LXR on cell cycle regulators by RT-qPCR (A) and Western blot (B).</b> HepaRG cells have been treated 24h in the same condition as for the microarrays (same ligands and DMSO as control). (A) Relative mRNA levels for genes regulated in the microarray data. (B) Representative Western blot of Retinoblastoma (Rb), phospho-Rb, Cyclin D3, and p27 in whole HepaRG cell lysates. (C) densitometric quantification of phospho-Rb and Rb. *: p-value < 0.05, ** < 0.01 versus DMSO (Student’s t test). N = 3.</p>