10.1371/journal.pone.0220894.g005
Leonore Wigger
Leonore
Wigger
Cristina Casals-Casas
Cristina
Casals-Casas
Michaël Baruchet
Michaël
Baruchet
Khanh B. Trang
Khanh B.
Trang
Sylvain Pradervand
Sylvain
Pradervand
Aurélien Naldi
Aurélien
Naldi
Béatrice Desvergne
Béatrice
Desvergne
System analysis of cross-talk between nuclear receptors reveals an opposite regulation of the cell cycle by LXR and FXR in human HepaRG liver cells - Fig 5
Public Library of Science
2019
GW 3965
GW 4064
Global gene expression
LXR
pro-proliferative effect
NR activities
target genes
liver cells
HepaRG liver cells Transcriptional regulations
4 hours
agonist
glucose metabolism
system analysis
24 hours
receptor outcome
NR specificity
FXR ligand
cell line HepaRG
CDCA
hepatocyte differentiation
PPAR
hepatocyte cells division
HepaRG cells
cell cycle
cell cycle progression
2019-08-22 17:38:53
Figure
https://plos.figshare.com/articles/figure/System_analysis_of_cross-talk_between_nuclear_receptors_reveals_an_opposite_regulation_of_the_cell_cycle_by_LXR_and_FXR_in_human_HepaRG_liver_cells_-_Fig_5/9720371
<p><b>Validation of the effects of FXR and LXR on cell cycle regulators by RT-qPCR (A) and Western blot (B).</b> HepaRG cells have been treated 24h in the same condition as for the microarrays (same ligands and DMSO as control). (A) Relative mRNA levels for genes regulated in the microarray data. (B) Representative Western blot of Retinoblastoma (Rb), phospho-Rb, Cyclin D3, and p27 in whole HepaRG cell lysates. (C) densitometric quantification of phospho-Rb and Rb. *: p-value < 0.05, ** < 0.01 versus DMSO (Student’s t test). N = 3.</p>